Effect of Tryptophane on Synthesis of Certain Exometabolites by Bacteria of Genus Acinetobacter, Nocardia, and Rhodococcus and Their Properties

The efficiency of integrated microbial biotechnologies for obtaining several practically valuable metabolites in one technological process is determined both by the maximum concentration these substances and their properties. This especially true secondary metabolites, composition properties which vary depending on cultivation conditions producer. Aim. To research effect tryptophan (a precursor auxin biosynthesis) culture media synthesis certain exometabolites Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus B-7241, Nocardia vaccinii B-7405 as well Methods. R. A. B-724, N. were cultivated a medium containing refined waste sunflower oil, biodiesel waste, or ethanol carbon source. was 300 mg/L. Surfactants extracted from supernatant cultural liquid with modified Folch mixture. Phytohormones isolated sequential extraction organic solvents after surfactant extraction. Thin-layer chromatography used preliminary purification phytohormones. Qualitative quantitative determination auxins performed using high-performance chromatography. antimicrobial activity surfactants analysed minimum inhibitory concentration. enzymes surface-active glycoand aminolipids biosynthesis (phosphoenolpyruvate synthetase, phosphoenolcarboxykinase, NADP+-dependent glutamate dehydrogenase) spectrophotometrically during oxidation NADH NADP. Results. It found that presence strains under study did not affect number synthesized surfactants, 1.80−1.90, 1.55−1.75, 1.50−1.65 g/L, respectively. At same time, increased phytohormones: it higher than amount phytohormones without precursor. introduction into accompanied formation surfactants. These compounds showed 2−4 times against phytopathogenic bacteria (Agrobacterium tumefaciens UCM B-1000, Pseudomonas syringae B-1027T, Xanthomonas vesicatoria B-1106, Pectobacterium carotovorum B-1075T, Clavibacter michiganensis B-102 pv. tomato B-9167) B-7241 either change compared to obtained tryptophan, slightly. Data correlated indicators activity. In dehydrogenase cells key enzyme responsible activity) almost 1.4 tryptophan-free medium. Conclusions. As result this work, researched remained unchanged established biosynthesis. data testify high potential use complex preparations crop production stimulate growth plants biocontrol bacteria.